| Detecting Gene Expression in Drosophila |
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One of the problems in biology is understanding the process of development in higher organisms. Many workers are using the fruit fly, Drosophila, to study development. Fruit flies contain much less DNA than the mouse, making it easier to identify genes that are important for normal development. One method that has been developed recently and has proven to be especially effective involves the use of the green fluorescent protein (GFP) to 'report' the activity of a yeast gene, gal4, introduced into Drosophila. Using the Leica fluorescence stereomicroscope, the follicle cell fluorescence can be observed not only in newly dissected ovaries, but also through the abdominal cuticle of intact live Drosophila adult females. The ability to observe the patterns of GFP fluorescence in intact live Drosophila will greatly simplify screening of gal4 insertion lines for new patterns of fluorescence to identify further genes expressed in a developmentally regulated fashion in the follicle cells.
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