Leica LAS Power Mosaic

Overview

LAS Power Mosaic provides the ultimate in high-resolution specimen visualisation. Scan the entire specimen or select a specific region of interest and it's scanned at high speed. The captured images are immediately combined to form a seamless mosaic image. Once the scan is complete you can relocate effortlessly to areas of interest with a single mouse click and can view the live microscope image that corresponds to a chosen location. Additionally you can zoom and pan around an entire digitised mosaic image using easy and intuitive browsing tools. LAS Power Mosaic is ideal for both routine and research applications and provides modes of scanning to suit all forms of optical microscopy. In addition to the features of LAS Power Mosaic, the plus edition further extends the capability using Z-Stack acquisition to provide 3-D mosaic imaging and visualisatione.

Key Features

• LAS Power Mosaic is suitable for a diverse range of applications, both research and routine. Due to the ability to provide appropriate scanning methods for different microscopy fields, the system has great versatility and is used successfully in many different environments. Features include:
• Scanning and acquisition at camera frame rates to ensure high speed and rapid throughput
• Tile edge merging provides highest possible image quality
• No restrictions on objective or imaging method
• Fast and accurate relocation with easy and intuitive review tools
• Intelligent memory management supports mosaic sizes limited only by available disk space
• Fully automated focus control and tracking
• User control of image size to ensure suitability for publication and archiving
• One click calibration for easy camera alignment
• Optional advanced functions, including powerful 3-D Z-Stack mosaic acquisition for powerful focus visualisation

LAS Power Mosaic Detailed Description

When the system is initially configured a simple, single click calibration procedure is performed for each objective lens on the microscope. This informs the system of the exact correlation between a stage movement and the associated spatial value in microns for the objective. This calibration information enables the system to maintain precise and accurate positional information for each image acquired.In particular, this also compensates for camera rotation and stage orientation.

In addition to the spatial calibration of the system, "shading correction" is set-up to eliminate any uneven illumination in the acquired images. You can also employ a number of different automatic focussing techniques as appropriate for the scanning method and the nature of the specimen.

Once the scan area has been defined the system automatically calculates the number of images that need to be acquired to form a complete full resolution mosaic of the chosen area. You simply select the type of scan required. The exact nature of the scan performed is fully controllable and depends on the microscopy method employed and the format of mosaic required. Everything else is handled automatically by the system, using previously defined values to determine speed, focussing method and data format.

When the scan is complete, you may revisit points of interest with a single mouse click or copy all or part of the mosaic for distribution, publication or discussion with colleagues. Additionally, since the entire area scanned is now available in digital form and at full resolution, the image data is available for annotation or further analysis within applications active in the Leica Application Suite,

LAS Power Mosaic requires a Leica motorised microscope, Leica Digital Camera, motorised stepped stage, motorized focus with XY control board, a high performance Windows PC and a very large hard drive.